by the activities of dehydrogenases in cells, is directly proportional to the number of living cells. Cell Counting Kit-8 (CCK-8) allows sensitive colorimetric assays promo code lamingtondrive for the determination of cell viability in cell proliferation and cytotoxicity assays. To ensure the data is reflecting the cell death instead of decreased metabolic activity, Cytotoxicity LDH Assay Kit-WST (. What other filters can I use?
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Cell viability detection mechanism with CCK-8. CCK-8 is stable over 6 months at room temperature and one year at 0-5 oC with protection from light. Since its formazan dye is also highly water soluble, CCK-8 cannot be utilized for cell staining. However, please note that since CCK-8 uses a different assay mechanism from that of the code promo pg plastique Thymidine assay, so the results may differ. For leukocytes, at least 2,500 cells are necessary per well (100 l medium) because of low sensitivity. Since the toxicity of CCK-8 is so low, the same cells can be used for other cell proliferation assays such as the crystal violet assay, neutral red assay or DNA fluorometric assay after the CCK-8 assay is completed. Comparison of the sensitivity using tetrazolium dye. It is also stable at 4C for 1 year.
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